Product Description:

• Endotoxin-free
• Animal-derived product free
• Lyophilized and Carrier Free (CF)
• High Activity

Xeno-free human IL-28A^HuXp (IFN lambda 2) is expressed in human 293 cells with an apparent molecular mass of 24 kDa. This cytokine is produced in a serum-free, chemically defined media. Production in human 293 cells offers authentic human post-translational modification which contributes to stability in cell growth media and other applications. The purity is greater than 95%.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤5 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/μg
• Molecular Mass: 24 kDa, monomer, non-glycosylated
• Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose-dependent protection of the cytopathic effect on A549 cells (human lung adenocarcinoma epithelial cell line) that were challenged with encephalomyocarditis (EMC) virus.

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

HumanZyme

HumanZyme公司是全球领先的提供高度真实的重组人细胞因子和生长因子从人类细胞表达。HumanZyme利用其专有的工程人类细胞系、表达载体和细胞培养介质，以确保高产量的重组蛋白，包括糖基化、二硫键和亚基组装等天然人类翻译后修饰。

HumanZyme, Inc. is the global leader in providing highly authentic recombinant human proteins from human cells. Our process is cost-effective and scalable making our proteins suitable for the research, diagnostic, drug discovery and biopharmaceutical markets. HumanZyme leverages its proprietary engineered human cells, expression vector, and cell culture media to ensure high-yield production of recombinant proteins with native human post-translational modifications, such as disulfide bonds and glycosylation. We are a leading provider of cytokines and growth factors and a preferred outsourcing supplier of human protein production. Our products and services support a broad range of customers worldwide–from academic and government research institutions to biotechnology and pharmaceutical companies.