Product Description:

  • Endotoxin-free
  • Animal-derived product free
  • Lyophilized and Carrier Free (CF)
  • High Activity

Xeno-free IL-9HuXp is expressed from human 293 cells as a glycoprotein monomer with apparent molecular mass of 38 to 48 kDa. IL-9 is produced in a human cell expression system with serum-free, chemically defined media. It stimulates cell proliferation and also prevents apoptosis. IL-9 acts as a regulator for a variety of hematopoietic cells. It enhances the expansion and recruitment of mast cells and eosinophils. This cytokine is greater than 95% pure.

Technical Specifications:

  • Species: Human
  • Expression: HEK293 Cell Expressed
  • Activity: Typically ≤ 1 ng/mL EC50
  • Purity: >95%
  • Endotoxin: <1 EU/μg
  • Molecular Mass: 38 to 48 kDa, monomer, glycosylated
  • Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose-dependent stimulation of the proliferation of human MO7e cells (human megakaryoblastic leukemia cell line).

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).