Product Description:
- Endotoxin-free
- Animal-derived product free
- Lyophilized and Carrier Free (CF)
- Authentic Glycosylation
Xeno-free SCFHuXp is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 35 to 45 kDa. This product is produced in a human cell expression system with serum-free, chemically defined media. Production in human 293 cells offers authentic glycosylation, contributing to stability in cell growth media and other applications. SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation, and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages.
Technical Specifications:
- Species: Human
- Expression: HEK293 Cell Expressed
- Activity: Typically ≤25 ng/mL EC50
- Purity: >95%
- Endotoxin: <1 EU/µg
- Molecular Mass: 35 to 45 kDa, monomer, glycosylated
- Formulation: 1x PBS
Purity Confirmation:
The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
Activity Assay:
The activity was determined by the dose- dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
Reconstitution Buffer:
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).