Product Description:

  • Endotoxin-free
  • Animal-derived product free
  • Lyophilized and Carrier Free (CF)
  • Authentic Glycosylation

Xeno-free SCFHuXp is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 35 to 45 kDa. This product is produced in a human cell expression system with serum-free, chemically defined media. Production in human 293 cells offers authentic glycosylation, contributing to stability in cell growth media and other applications. SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation, and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages.

Technical Specifications:

  • Species: Human
  • Expression: HEK293 Cell Expressed
  • Activity: Typically ≤25 ng/mL EC50
  • Purity: >95%
  • Endotoxin: <1 EU/µg
  • Molecular Mass: 35 to 45 kDa, monomer, glycosylated
  • Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose- dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).