Product Description:
- Endotoxin-free
- Animal-derived product free
- Lyophilized and Carrier Free (CF)
- Authentic Glycosylated Homodimer
Xeno-free VEGF 165is expressed in human 293 cells as a homodimeric glycoprotein with an apparent molecular mass of 45 kDa. The E. coli expressed protein is a monomer-dimer mixture with a molecular mass 18 to 34 kDa. The cytokine is produced in a human cell expression system with serum free, chemically defined media. VEGF, also known as vascular permeability factor (VPF) or vasculotropin, is a homodimeric 34-45 kDa, heparin-binding glycoprotein with potent angiogenic, mitogenic, and vascular permeability enhancing activities specific for endothelial cells.
Technical Specifications:
- Species: Human
- Expression: HEK293 Cell Expressed
- Activity: Typically ≤15 ng/mL EC50
- Purity: >95%
- Endotoxin: <1 EU/µg
- Molecular Mass: 45 kDa, homodimer, glycosylated
- Formulation: lx PBS
Purity Confirmation:
The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
Activity Assay:
The activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (Human Umbilical Vein Endothelial Cells).
Reconstitution Buffer:
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant serum albumin (HSA).